Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-12-20
1992-12-01
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435 79, 435 792, 435961, 436534, 436174, 436 63, 436548, G01N 3353, G01N 33577
Patent
active
051680440
ABSTRACT:
Immunodiagnostic assays for the detection and determination of mastitis and sub-chemical mastitis comprise capturing neutrophils or fragments or soluble products thereof in a milk sample on an insolubilized form of a corresponding antibody, optionally using conditions whereby the cells in the milk sample are lysed. Monoclonal antibodies are provided which can be used in said assays and which are specific to neutrophils.
REFERENCES:
Nickerson et al, "Production of Monoclonal Antibodies to Bovine Leukocyte Cell-Surface Components" Journal of Dairy Science, 66(7) pp. 1547-1558 (1983).
Lostrie-Trussart et al, "Phagocytic Cells of Cow's Milk: Identification with Monoclonal Antibodies and Functional Study in vitro", Annales de Medecine Veterinaire, 131(1) pp. 49-62 (1987).
Sevier et al, "Monoclonal Antibodies in Clinical/Immunology", Clin. Chem 27(11) pp. 1797-1806 (1981).
Ball et al., J. Clin. Microbiol., 29(8):1625-1628 (Aug. 1991).
International Dairy Federation Annual Bulletin, "A Monograph on Bovine Mastitis-Part I" pp. 3-5 (1971).
Darnell et al, eds., Molecular Cell Biology pp. 157 and 166.
Aldrich Chemical Company, Inc. catalog p. 355 (1990).
Joyce Patrick J.
O'Sullivan Catherine A.
Shattock Alan G.
Sloan Teresa M.
Bidwell Carol E.
Kepplinger Esther L.
University College Dublin
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