Immunodeficiency virus neutralization assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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4352401, 4352402, 43524021, 435974, C12Q 170

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054748941

ABSTRACT:
A method for assaying the ability of an antibody to neutralize primary clinical isolates of human immunodeficiency virus (HIV) in primary human lymphocytes comprising the steps of contacting primary human lymphocytes with HIV and an antibody sample to be tested, without preincubating the antibody sample and the virus, culturing the primary human lymphocytes under conditions which allow for HIV replication without removal of the virus inoculum or antibody samples to be tested, and measuring HIV replication in the primary human lymphocytes by one or more of several conventional methods.

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