Immunochromatographic assay with support having bound "MIP" and

Details Immunochromatographic assay with support having bound "MIP" and Immunochromatographic assay with support having bound "MIP" and

1982-07-15

1984-03-06

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

210658, 422 56, 435 4, 435 25, 435 28, 435805, 436162, 436514, 436530, 436541, 436810, 436815, G01N 3354, G01N 3352, G01N 3108, G01N 3358

Patent

active

044355041

ABSTRACT:
Chromatographic immunoassay employing a specific binding pair member and a label conjugate which delineates a border whose distance from one end of the chromatograph relates to the amount of analyte present. By combining the label conjugate and sample in a solution and immunochromatographing the solution, or employing a combination of enzymes, one enzyme being the label and the other enzyme affixed to the chromatographic support, the position of the border defined by the label can be related to the amount of analyte in the sample solution.
Preferably, an immunochromatograph is employed having both a specific binding pair member and an enzyme affixed to the support. A sample is chromatographed and the amount of analyte is determined by (1) contacting the chromatograph with a second enzyme conjugated with a specific binding pair member which binds to the chromatograph in proportion to the amount of analyte bound to the chromatograph, or (2) including the second enzyme conjugate with said sample, resulting in a defined border related to the amount of analyte in the sample. The two enzymes are related in that the substrate of one is the product of the other, so that upon contact of the chromatograph with appropriate reagents, a detectable signal develops which permits detection of the border to which the analyte traveled. This distance can be related to the amount of analyte present in the sample.

REFERENCES:
patent: 4168146 (1979-09-01), Grubb
patent: 4205058 (1980-05-01), Wagner
patent: 4298688 (1981-11-01), Kallies
C. Glad et al., Anal. Biochem., 85, 180-187 (1978).
C. Glad et al., Anal. Biochem., 116, 335-340 (Sep. 15, 1981).

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