Immunochemical LDH.sub.1 assay

Details Immunochemical LDH.sub.1 assay Immunochemical LDH.sub.1 assay

1978-02-27

1980-09-23

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

424 12, 435 26, C12Q 166, C12Q 132

Patent

active

042244064

ABSTRACT:
LDH.sub.1 levels in serum samples can be rapidly and accurately assayed by a novel immunochemical technique. In such procedure the serum sample is treated with soluble antibodies against the M subunit and the resulting antigen-antibody reaction product is insolubilized with a second antibody supported on an insoluble material. The resulting supernatant containing only LDH, isoenzyme is assayed for enzymatic activity by conventional procedures. The assay of LDH.sub.1 levels in sera is useful in determining whether the subject has undergone a myocardial infarction.

REFERENCES:
patent: 3932221 (1976-01-01), Pfleiderer
patent: 4012285 (1977-03-01), Pfleiderer et al.
patent: 4046634 (1977-09-01), Mercer
patent: 4048298 (1977-09-01), Niswender
Murket, et al., "Immunochemical Properties of Lactate Dehydrogenase Isoenzymes", Ann. N.Y. Acad. Sci., vol. 103 (1963), pp. 915-929.
Sussmann, et al., "Human Alkaline Phosphatase", J. Biol. Chem., vol. 243, No. 1 (1968), pp. 160-166.
Boll, et al., "Quantitative Immunological Determination of Multiple Forms of the Lactate Dehydrogenase in Animal and Human Tissue", Hoppe-Seyler's Z. Physiol. Chem., vol. J55 (1974), pp. 811-818.
Clark, et al., "A Simple and Rapid Procedure for the Preparation of Human LDH-1 and LDH-5 Using an Ion-Exchange-Column", Chem. Abst., vol. 85, No. 7 (1976), p. 217, abs. #42732a.

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