Immunochemical displacement for determining an analyte

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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422 56, 435 793, 435 794, 435970, 436501, 436514, 436518, G01N 33543, G01N 33558

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active

055739210

ABSTRACT:
The present invention pertains to a process for determining an analyte, especially a low-molecular-weight pollutant in a sample. It is carried out by partially dipping into the sample a test strip, on which antibodies which are specific for the analyte and to which in turn a tracer is bound, are immobilized. During the capillary migration of the sample through the test strip, the analyte partially displaces the tracer. The tracer that has remained on the antibody is subsequently visualized by, e.g., dyeing. The intensity of the dyeing is an indicator of the amount of analyte detected.

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A. J. Pesce et al, Scand. Journ. Immunol. 8, Suppl. 7, 1-6, 1978.

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