Immunochemical determination of multivalent analytes

Details Immunochemical determination of multivalent analytes Immunochemical determination of multivalent analytes

1998-06-04

2000-09-19

Wortman, Donna C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 732, 436518, C12Q 170, C12Q 104

Patent

active

061209902

ABSTRACT:
The present invention is directed to an immunochemical method for qualitatively or quantitatively detecting an analyte in which a first and a second binding component are contacted with the analyte, followed by detection of the binding of the binding components to the analyte. According to the invention, both of the binding components are prepared recombinantly in the same host using different vectors (V1 and V2) for expressing fusion proteins, with the first binding component being expressed as a fusion protein F1 in vector V1 and the second binding component being expressed as a fusion protein F2 in vector V2. The novel method, which can also be employed for simultaneously determining several analytes, is more sensitive and specific than prior known methods.

REFERENCES:
patent: 5322769 (1994-06-01), Bolling et al.
patent: 5480972 (1996-01-01), Avgioglu et al.
patent: 5496934 (1996-03-01), Shoseyov et al.
Knapp et al., Biotechniques, A Simple Water Level Detection Device, vol. 8, No. 3 (1990), p. 281.

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