Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-12-03
1996-05-14
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 721, 435 792, 43524026, 436 63, 436 64, 436518, 5303877, 5303879, 5303888, 53038885, G01N 3353, G01N 33574
Patent
active
055166431
ABSTRACT:
Disclosed are antibodies to peptides active in the structuredness of the cytoplasmic matrix test (SCM-factor peptides) and to fragments of the peptides by immunization of antibody-producing animals with the peptides or fragments. Both polyclonal and monoclonal antibodies can be prepared. Particularly useful are antibodies specifically binding the peptides M-I-P-P-E-V-K-F-N-K-P-F-V-F-L-M-I-D-Q-N-T-K-V-P-L-F-M-G-K (SEQ ID NO: 2) and F-L-M-I-D-Q-N-T-K (SEQ ID NO: 3). The antibodies can be labeled and are suitable for performing immunoassays to detect the presence of SCM cancer-recognition factors in cell cultures or body fluids. One particularly useful immunoassay can distinguish SCM factor from partially homologous peptide sequences, and comprises: (a) incubating a first aliquot of the sample with a first antibody specific for the cancer-recognition factor to bind the first antibody to the cancer-recognition factor in the first aliquot; (b) reacting a second aliquot of the sample with a second antibody specific for the amino-terminal portion of the partially homologous peptide sequence to bind the second antibody to the partially homologous peptide sequence in the second aliquot; and (c) comparing the quantity of the first antibody bound to the first aliquot with the quantity of the second antibody bound to the second aliquot to detect the cancer recognition factor.
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C. Longstaff & P. J.
Cercek Boris
Cercek Lea
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