Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-05-30
1999-09-07
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 436507, 436518, 436523, 436533, G01N 3353
Patent
active
059486279
ABSTRACT:
The present invention provides an improved method for detection of panel reactive antibodies in serum of a subject against HLA class I antigens, which comprises the steps of adding serum from a subject to an array of microbeads, each microbead presenting HLA antigens from a cell population presenting the same HLA antigens; incubating the serum and microbeads for sufficient time for anti-HLA antibodies in the serum to bind to the HLA antigens presented on the microbeads; removing the serum components which do not specifically bind with the HLA antigens presented on the microbeads; incubating the microbeads with a labeled ligand capable of specifically binding with anti-HLA antibodies bound to said HLA antigens; removing the labeled ligand which is not bound to said HLA antigens; and detecting the presence of labeled ligand bound to said HLA antigens by flow cytometry.
REFERENCES:
patent: 4918000 (1990-04-01), Schubert
patent: 5223397 (1993-06-01), Pouletty et al.
patent: 5514557 (1996-05-01), Moghaddam
patent: 5567627 (1996-10-01), Lehnen
A. Nanni-Costa et al., "ELISA anti-HLA antibody screening identifies non-complement-fixing antibodies responsible for acute graft rejection. A case report." EUROPEAN JOURNAL OF IMMUNOGENETICS, 23(5):383-387, Oct. 1996.
A. Zachary et al., "Evaluation of HLA antibodies with the PRA-STAT test." TRANSPLANTATION, 60(12):1600-1606, Dec. 1995.
ATCC Catalogue of Cell Lines & Hybridomas, 7th edition, pp. 264, 323-324, 521, 1992.
Harmer et al., "Detectionof HLA Class I-and Clas 5 II-Specific Antibodies by Flow Cytometry and PRA-Stat Screening in Renal Transplant Patients," TRANSPLANTATION, 63(12):1828-1832, Jun. 1997.
Kao et al., "Enzyme-Linked Immunoassay for Anti-HLA Antibodies --An Alternative to Panel Studies by Lymphocytoxicity", TRANSPLANTATION, 55(1):192-196, Jan. 1993.
Kohler et al., "Flow cytometric detection of platelet-reactive antibodies and application in platelet crossmatching", TRANSFUSION, 36(3):250-255, Mar. 1996.
Massad et al., "Factors Influencing HLA Sensitization in Implantable LVAD Recipients", ANNALS OF THORACIC SURGERY, 64:1120-1125, Oct. 1997.
Parham, P., "Purification of Immunologically Active HLA-A and -B Antigens by a Series of Monoclonal Antibody Columns", THE JOURNAL OF BIOLOGICAL CHEMISTRY, 254(18):8709-8712, Sep. 1979.
Walker et al., "Human Histocompatibility Antigens: Isolation and Chemical Characterization", JOURNAL OF IMMUNOLOGICAL METHODS, 49:R25-R50, 1982.
Cantarero et al., Anal. Biochem.105: 375-382 (1980).
Frengen et al., Clin. Chem.40/3: 420-425 (1994).
Henderson et al., Virology76: 152-163 (1977).
Scillian et al., Blood73: 2041-2048 (1989).
Shroyer et al., Transplantation59: 626-630 (1995).
Sumitran-Karuppan et al., Transplantation61: 1539-1545 (1996).
Wilson et al., J. Immunol. Methods107: 231-237 (1988).
Zaer et al., Transplantation63: 48-51 (1997).
Lee Jar-How
Pei Rui
One Lambda
Spiegel Carol A.
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