Immunoassays using antigens produced in heterologous organisms

Details Immunoassays using antigens produced in heterologous organisms Immunoassays using antigens produced in heterologous organisms

1992-07-29

1993-10-19

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 794, 435 71, 435 792, 435973, 435974, 436820, G01N 3353

Patent

active

052544583

ABSTRACT:
This invention relates to improved "sandwich" immunoassays for antibodies in body fluids of the type where antigen specific for the antibody to be detected is disposed on a solid support and binds antibody from the body fluid, from which the antibody bound to the solid support is detected by a labeled antigen to the antibody to be detected. The improvement comprises using antigens from heterologous cell sources.

REFERENCES:
patent: 4774175 (1988-09-01), Chang et al.
Odell et al: Principles of Competitive Protein-Binding Assays John Wiley & Sons, Inc. 1983, pp. 246-249.
Crowl, et al Cell 41, 979-986, 1985 (II).
Voller, et al In: Manual of Chemical Laboratory Immunology 3rd Edition, Editing: Rose, et al, 1986.

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