Immunoassays involving complement lysing of chromophore containi

Details Immunoassays involving complement lysing of chromophore containi Immunoassays involving complement lysing of chromophore containi

1984-06-08

1987-12-22

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436517, 436520, 436522, 436805, 436821, 436829, G01N 3353, G01N 33555

Patent

active

047146729

ABSTRACT:
An immunoassay of the type which utilizes the hemolysis of microcapsules such as red blood cells. Microcapsules which can be lysed by complement activity, which contain an optically determinable substance, on whose surfaces an antibody to be quantified is bound are prepared. The microcapsules, a test sample containing the antigen or the antibody to be quantified, and complement are mixed to react each other. Thereafter, an optical measurement is conducted at different wavelengths for the reaction mixture which is still suspending the intact microcapsules.

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Chemical Abstract, vol. 87, No. 3, 18th Jul. 1977, p. 427, No. 20388g, Columbus, Ohio, Smolarsky et al., "A Simple Fluorescent Method to Determine Complement-Mediated Liposome Immune Lysis" & J. Immunol. Methods 1977, 15(3), 255-65.
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D'Orzio et al., Ion Electrode Measurements of Complement and Antibody Levels Using Marker-Loaded Sheep Red Blood Cell Ghosts, Anal. Chem, vol. 49, No. 13, Nov. 1977, pp. 2083-2086.

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