Immunoassays in capillary tubes

Details Immunoassays in capillary tubes Immunoassays in capillary tubes

1996-07-29

1999-11-02

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436 20, 436 23, 436172, 436538, 422 57, 422 681, 422 8207, 422 8208, G01N 3302

Patent

active

059768967

ABSTRACT:
A fluorescent immunoassay employing the interior surface of a capillary tube is provided. Devices to permit immunoassays using one or more capillary tubes, an apparatus for use with the devices, and a process for screening for analyte in a sample using the devices and apparatus are also provided. Samples suspected of containing analyte are added to a disposable self-contained sample tray containing one or more sample wells, mixed with a reagent, drawn into one or more spaced-apart capillary tubes held within a disposable cartridge connected to an analytical apparatus, reacted with a binding member on the surface of the capillary tube, washed to stop the reaction, and dried by the apparatus. The capillary tube is then exposed to a signal generation device to create a fluorescence signal that is detected using a signal detector. The apparatus determines the presence of the analyte and optionally determines the amount of analyte present in the sample, and presents the results to the operator.

REFERENCES:
patent: 4116638 (1978-09-01), Kenoff
patent: 4590157 (1986-05-01), Chandler et al.
patent: 4716121 (1987-12-01), Block et al.
patent: 5624850 (1997-04-01), Kumar et al.

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