Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1994-06-06
1997-04-29
Housel, James C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436 20, 436 23, 436172, 436538, 422 57, 422 681, 422 8207, 422 8208, G01N 3302, G01N 33552, G01N 2100, G01N 1506
Patent
active
056248504
ABSTRACT:
Immunoassay methods employing capillary containers are provided. The immunoassays may be competitive or sandwich immunoassays, where fluorescently labeled conjugates are used. Sample suspected of containing analyte is combined with fluorescently labeled conjugate, as well as any additional reactants which may be required. At least a portion of the incubated sample is placed in a capillary container. The capillary container has, on at least one region of its inner surface, a binding member capable of complexing with the fluorescently labeled conjugate, either directly or indirectly. Fluorescently labeled conjugate not complexed to binding member is then washed from the capillary container. The complexed fluorescently labeled conjugate is detected by irradiating the capillary and measuring the emitted signal. The intensity of the emitted signal is indicative of the presence of analyte in the sample.
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Deshpande Sudhir
Kharadia Shanta
Kumar Amit
Piani Marcos
Rocco Richard
Housel James C.
Idetek, Inc.
Shaver Jennifer
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