Immunoassays for thyroid hormones using thyroglobulin

Details Immunoassays for thyroid hormones using thyroglobulin Immunoassays for thyroid hormones using thyroglobulin

1990-10-17

1993-03-23

Naff, David M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

435 792, 435 793, 436518, 436523, 436526, 436534, 530386, G01N 3353, G01N 33543, G01N 33553

Patent

active

051963493

ABSTRACT:
Immunoassays for measuring thyroid hormones such as thyroxine(T.sub.4) and triiodothyronine(T.sub.3) are carried out by incubating a serum sample with either (a) unlabeled thyroglobulin and a labeled antibody to a thyroid hormone or to thyroxine binding globulin(TBG), or (B) labeled thyrogloublin and an unlabeled antibody to a thyroid hormone. The thyroglobulin may be immobilized on an insoluble carrier and the immobilized thyroglobulin is preferably modified by succinylation with succinic anhydride. Whole thyroglobulin can be used or the thyroglobulin can be fragmented into smaller peptides prior to use provided the peptides contain at least one T.sub.3 or T.sub.4 residue per peptide. Preferably, the antibody is a monocional antibody and labeling is with an acridinium ester.

REFERENCES:
patent: Re32098 (1986-03-01), Chopra
patent: 4410633 (1983-10-01), Hertl et al.
patent: 4824777 (1989-04-01), Cheng et al.
patent: 4966838 (1990-10-01), Ferrua et al.
Tao, et al., Chemical Abstracts, 94:61029d, 1981.

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