Immunoassays for prostate specific antigen

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 794, 435967, 435975, 436507, 5303911, G01N 33531, G01N 33573

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active

056724802

ABSTRACT:
The present invention relates to the field of cancer immunoassay. Specifically, a new immunoassay method for prostate specific antigen (PSA) is presented. Also presented is a complex which resembles a complex of PSA and .alpha.-antichymotrypsin (ACT) that can be used as a calibrator or control in an immunoassay for PSA. Further presented is a method for fractionating polyclonal antibodies, to PSA, into those which bind epitopes that are masked by the binding of PSA to ACT and those which do not bind such epitopes.

REFERENCES:
patent: H1018 (1992-01-01), Hantke et al.
patent: 5223441 (1993-06-01), Ullman et al.
patent: 5501983 (1996-03-01), Lilja et al.
"Immunoaffinity Chromatography with Affinity Supports", Bio-Rad Bulletin 1099, (1990).
H. Lilja et al., "Prostate Specific Antigen Predominantly Forms a Complex with Alpha.sub.1 -Antichymotrypsin in Blood", Cancer Supplement, 70 (1992), pp. 230-234.
K. Pettersson et al, "Development of Immunoflourometric Methods for PSA to Improve the Discrimination Between Early Prostate Cancer and Benign Prostatic Hyperplasia" XV International Congress of Clinical Chemistry (1993).
O. Nilsson et al., "Epitope mapping of PSA, and development of assays for the determination of different isoforms of PSA", CanAg Diagnostics AB, (1993).
"Epitope mapping of PSA, and development of assays for the determination of different isoforms of PSA", Abstract P38, Journal Tumor Marker Oncology, (1993)10th Int'l Conference on Human Tumor Markers.
W.G. Wood et al., "The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Specific Antigen-.alpha..sub.1 -Antichymotrypsin Complexes in Serum", European Journal Clinical Chemistry Clinical Biochemistry, 29(1991), pp. 787-794.
Pharmacia LKB Biotechnology, "Coupling gels for ligand immobilization", Affinity Chromatography principales & methods, (1993)pp. 23-30.
A. Christensson et al., "Serum Prostate Specific Antigen Complexed to .alpha.1-Antichymotrypsin as an indicator or Prostate Cancer", Journal of Urology, 150(1993), pp.100-105.
U. Stenman et al., "A Complex between Prostate-specific Antigen and .alpha.1-Antichymotrypsin Is the Major Form of Prostate-specific Antigen in Serium of Patients with Prostatic Cancer: Assay of the Complex Improves Clinical Sensitivity for Cancer" Cancer Research, 51(1991) pp. 222-226.
H. Lilja et al., "Prostate-Specific Antigen in Serum Occurs Predominantly in Complex with .alpha.1-Antichymotrypsin", Clinical Chemistry, 37,(1991) pp.1618-1625.
B. Bluestein et al., "Multi-Site Clinical Evaluation of an Automated Chemiluminescent Immunoassay for Prostate Specific Antigen (ACS.TM.PSA)", Journal of Tumor Marker Oncology, 7(1992), pp. 41-60.
K. Watt et al., "Human prostate-specific antigen: Structural and functional similarity with serine proteases", Proc. Natl. Acad. Sci. USA, 83(1986), pp. 3166-3170.
Y. Ban et al., "The Proteolytic Activity of Human Prostate-Specific Antigen", Biochemical and Biophysical Research Communications, 123 (1984), pp. 482-490.
T.A. Stamey et al., "Prostate-Specific Antigen as a Serum Marker for Adenocarcinoma of the Prostate", The New England Journal of Medicine, 317 (1987), pp.909-916.
M. Kurlyama et al., "Multiple Marker Evaluation in Human Prostate Cancer with the use of Tissue-Specific Antigens", J. Nat'l Cancer Inst., 68 (1982), pp.99-105.
P.H. Lange et al., "Serum Prostate-Specific Antigen: its use in Diagnosis and Management of Prostate Cancer" Supplement to Urology, 33 (Jun. 1989) pp. 13-17.
M.K. Brawer et al., "Prostate-Specific Antigen in Management of Prostatic Carcinoma", Supplement to Urology, 33(May 1989) pp. 11-16.
P.H. Lange et al., "The Value of Serum Prostate Specific Antigen Determinations Before and After Radical Prostatectomy", Journal of Urology, 141(1989), pp. 873-879.
T.A. Stamey et al., "Prostate Specific Antigen in the Diagnosis and Treatment of Adenocarcinoma of the Prostate.II. Radical Prostatectomy Treated Patients", The Journal of Urology, 141 (1989), pp. 1076-1083.
J.E. Oesterling et al., "Prostate Specific Antigen in the Preoperative and Postoperative Evaluation of Localized Prostatic Cancer Treated with Radical Prostatectomy", The Journal of Urology, 139(1988), pp. 766-772.
W.H. Scouten, "Affinity Chromatography for protein isolation", Current Opinion in Biotechnology, 2 (1991), pp. 37-43.
M. Flore, "The Abbott IMx.TM. Automated Benchtop Immunochemistry Analyzer System", Clinical Chemistry, 34 (1988), pp. 1726-1732.
M.J. Duffy, "New cancer Markers", Ann Clin Biochem, 26 (1989), pp. 379-387.
A. Lundwall, "Characterization of the gene for Prostate-specific antigen, a human glandular kallikrein", Biochemical and Biophysical Research Communicatications, 161(1989), pp. 1151-1159.
M. Kuriyama, "Quantitation of Prostate-specific Antigen in Serum by a Sensitive Enzyme Immunoassay", Cancer Research, 40 (1980) pp.4658-4662.
D.W. Chan, "Prostate-Specific Antigen as a Marker for Prostatic Cancer: a Monoclonal and a Polyclonal Immunoassay Compared", Clinical Chemistry, 33 (1987) pp. 1916-1920.
C.S. Killian, "Prognostic Importance of Prostate-specific Antigen for Monitoring Patients with States B.sub.2 to D.sub.1 Prostate Cancer", Cancer Research, 45(1985) pp. 886-891.
C. Schneider, "A One-step Purification of Membrane Proteins Using a High Efficiency Immunomatrix", The Journal of Biological Chemistry, 257(1982), pp. 10766-10769.
A. Johnstone, "Affinity chromatography and immunoprecipitation", Immunochemistry in Practice, Scientific Publications, Oxford, 1st Edition (1982) pp. 202-232.
T.H. Ji, "Bifunctional Reagents", Methods in Enzymology, 91(1983), p. 580.
SS. Wong, "Protein Chemical Cross-Linking", Biocatalyst Design for Stability and Specificity, Chp. 22, pp.266-282.
A. Barnes, "The Abbott IMx.RTM. and IMx Select.TM. Systems", Journal Clin. Immunoasssay, 2 (1991), pp. 115-119.
M.C. Wang, "Purification of a Human Prostate Specific Antigen", Investigative Urology, 17, pp.159-163.
J.K. Siddall, "An Evaluation of the Immunochemical Measurement of Prostatic Acid Phosphatase and Prostatic Specific Antigen in Carcinoma of the Prostate", Eur. Urol., 12(1986), pp.123-130.
T.A. Stamey, "Prostate Specific Antigen in the Diagnosis and Treatment of Adenocarcinoma of the Prostate. IV. Anti-Androgen Treated Patients", The Journal of Urology, 141(1989), pp. 1088-1090.
T.A. Stamey, "Prostate Specific Antigen in the Diagnosis and Treatment of Adenocarcinoma of the Prostate. III. Radiation Treated Patients", The Journal of Urology, 141(1989), pp. 1084-1087.
R.L. Lundblad, Chemical Reagents for Protein Modification, 2nd Edition, CRC Press, 1991.
M.N. Gupta, "Cross-Linking Techniques", Biocatalyst Design for Stability and Specificity, pp. 308-326.
M.M. Manson, ed., "Immunochemical Protocols" Methods in Molecular Biology, 10(1992), pp. 89-91.

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