Immunoassays for discriminating between brucellosis infections a

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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424 92, 435 34, 435101, 435174, 435810, 435822, 436501, 436518, 436543, 436808, 436809, 436811, 530350, 530812, 530825, G01N 3353, G01N 33543, G01N 33531, C12Q 104

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active

050064630

ABSTRACT:
A method is disclosed for discriminating between cattle vaccinated against and those infected with Brucella spp. The method involves immunoassay using a purified polysaccharide containing 4,6-dideoxy-4-acylamido-D-mannopyranosyl units obtained from B. abortus or from cross-reacting organisms, and results in improved differentiation between vaccinated and infected animals. Test kits are also disclosed for performing the assay and a process is disclosed for obtaining the O-chain polysaccharides in high purity and yield.

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