Immunoassays and kits for use therein which include low affinity

Details Immunoassays and kits for use therein which include low affinity Immunoassays and kits for use therein which include low affinity

1983-11-18

1986-06-17

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

435 4, 435 7, 435810, 436513, 436518, 436531, 436533, 436536, 436538, 436548, 436805, 436808, 436818, 436819, G01N 33546, G01N 3353, G01N 33543, C12Q 100

Patent

active

045956618

ABSTRACT:
An immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Ab.sub.a) to the Ag, and an antibody (Ab.sub.b) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Ab.sub.c) to the Ag, at least one different type of antibody (Ab.sub.d) bound to a solid carrier (SC.sub.1), and at least one different type of antibody (Ab.sub.e) to the Ag. The SC.sub.1 is selected from a group consisting of SC, at least one different solid carrier (SC.sub.2), and mixtures thereof (SC and SC.sub.2). Each type of L-Ab.sub.c, Ab.sub.d -SC.sub.1, and Ab.sub.e has a lower average affinity constant (K) for Ag than each respective K of L-Ab.sub.a, Ab.sub.b -SC and Ab.sub. e ; and the additional entity is present in an amount sufficient to avoid a hook effect.
Also, a reagent of the type comprising at least one first entity selected from a group consisting of L-Ab.sub.a, Ab.sub.b -SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Ab.sub.c and at least one different type of Ab.sub.d -SC.sub.1, and at least one different type of Ab.sub.e. Ab, L-Ab.sub.a, Ab.sub.b -SC, L-Ab.sub.c, Ab.sub.d -SC.sub.1, and Ab.sub.e are as defined above.

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