Chemistry: physical processes – Physical processes – Crystallization
Patent
1980-03-07
1982-11-23
Marantz, Sidney
Chemistry: physical processes
Physical processes
Crystallization
23915, 422 61, 424 1, 424 12, 427 2, 435 7, G01N 3354, G01N 3356
Patent
active
043603580
ABSTRACT:
A method for preparing an immunologically active coated solid phase, suitably in the form of a coated tube or a polymer latex, for solid phase immuno assays, in particular radio immuno assays, is disclosed. The method comprises coating a solid support which has an organic polymer surface with a coating solution containing the immunologically active agent, e.g., an antibody or antigen, a hydroxy lower alkyl amine, preferably tris(hydroxymethyl) amino methane, and a platelet substitute. The solid support can be a solid organic polymer, such as polystyrene, which can be coated directly with this coating solution. If the immunologically active agent has a molecular weight of below 20000, the organic polymer surface of the solid phase is a matrix of a gel forming hydrophilic polymer. In this case the platelet substitute and the hydroxylower alkyl amine can be omitted from the coating solution.
REFERENCES:
patent: 3395210 (1968-07-01), Lenahan
patent: 3880714 (1975-04-01), Babson
patent: 4069352 (1978-01-01), Parsons
patent: 4254096 (1981-03-01), Monthony
patent: 4256724 (1981-03-01), Rutner
patent: 4264571 (1981-04-01), Goldstein
patent: 4278651 (1981-07-01), Hales
Chemical Abstracts, 90: 18764v (1979).
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