Immunoassay with chromatographic medium and labelled reagent

Details Immunoassay with chromatographic medium and labelled reagent Immunoassay with chromatographic medium and labelled reagent

1984-02-22

1987-05-19

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

436500, 436518, 436533, 436536, 436541, G01N 33536, G01N 33543, G01N 33558, G01N 3378

Patent

active

046668632

ABSTRACT:
A method of performing an assay for an analyte comprises providing a chromatographic medium, preferably in sheet form, forming a reaction mixture containing a labelled reagent present partly in a form which is mobile on the medium and partly in a form which is immobile, the proportions of the two forms being related to the analyte concentration, applying the mixture to a spot on the chromatographic medium, optionally applying a solvent to cause the mobile form of the labelled reagent to migrate from the spot, and measuring the label intensity remaining at the spot. The method provides a convenient way of performing immunoassays, 2-site immunometric assays, and agglutination assays.

REFERENCES:
patent: 3843775 (1974-10-01), Wolf
patent: 4184849 (1980-01-01), Cambiaso
patent: 4205058 (1980-05-01), Wagner
patent: 4235601 (1980-11-01), Deutsch
patent: 4312854 (1982-01-01), Derfler
patent: 4435504 (1984-03-01), Zuk
patent: 4446232 (1984-05-01), Liotta

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