Immunoassay with an increasing cyclic detection system

Details Immunoassay with an increasing cyclic detection system Immunoassay with an increasing cyclic detection system

1983-12-08

1986-07-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 435 21, 435 26, 436518, 436536, G01N 3354, G01N 33543, C12Q 142, C12Q 132

Patent

active

045980420

ABSTRACT:
An immunoassay uses an antibody or antigen -enzyme conjugate which converts a precursor to a cycling agent used in a cycling detection system. The amount of cycling agent is continually increased permitting amplification. The prefered system converts NADP to NAD using the conjugate label and the cycling system interconverts NAD and NADH.

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Anggard et al., "Prostaglandins: Enzymatic Analysis" Science, 163, pp. 470-480 (1-31-1969).
O. H. Lowry, et al., "The Measurement of Pyridine Nucleotides by Enzymatic Cycling", J. Biological Chem., vol. 236, No. 10, 1060, pp. 2746-2755.
E. Pitkanen, "The Hydrolysis of Nicotinamide Adenine Dinucleotide Phosphate by Serum Alkaline Phosphatase", Enzyme, vol. 12, 1971, pp. 226-234.

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