Immunoassay utilizing two incubations with labeled antigen

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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Details

5303894, 435 71, 435 5, 435 28, G01N 3353, C12Q 170, C12Q 128, C07K 1600

Patent

active

061210064

ABSTRACT:
The present invention relates to an immunoassay for antibodies against an infectious agent. The present invention also relates to the use of the immunoassay and a kit for performing the immunoassay of the present invention. The immunoassay for antibodies against an infectious agent relates to a method comprising: i. incubating a sample suspected of containing said antibodies with immobilized antigen of the infectious agent and free labeled antigen of the infectious agent; ii. separating immobilized components from non-immobilized components; iii. incubating the immobilized components with further free labeled antigen of the infectious agent and removing non-immobilized components; and iv. determining the amount of labeled antigen immobilized, wherein the amount of label is indicative of the amount of said antibodies present in the sample.

REFERENCES:
patent: 4789628 (1988-12-01), Nayak
patent: 5683864 (1997-11-01), Houghton et al.

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