Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1979-10-25
1981-12-22
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 1, 424 8, 424 12, 23230B, G01N 3354
Patent
active
043071902
ABSTRACT:
A liquid, particularly but not exclusively a liquid of human origin such as blood serum or urine, is immunoassayed for antibodies, antigens or antibody: antigen complexes, using as a reagent in the analysis an active fraction from mouse ascitic fluid. This active fraction is a euglobulin and has the ability, like human C1q, to combine with antibody:antigen complexes but not with free antibody or antigen. Unlike human C1q, however, it remains active at high pH's and its activity is not destroyed by 0.1 M putrescine or 0.1 M hydrazine, so analyses on human body fluids can be carried out at high pH's or in the presence of putrescine and hydrazine, without interference from endogenous human C1q. The active fraction is a very broadly applicable reagent in immunoassays and particularly useful in techniques involving agglutination of latex particles.
REFERENCES:
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patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4138213 (1979-02-01), Masson et al.
patent: 4162895 (1979-07-01), Cambraso et al.
Van de Winkel et al., "Interference of Serum Glycoproteins in Radio Immunoassay of Rat Alfa-Fetoprotein", Chemical Abstracts, vol. 87, No. 19 (1977), p. 261, Abs. No. 148242x.
Cambiaso Cesar L.
Masson Pierre L.
Technicon Instruments Corporation
Tedesco S. P.
Wiseman Thomas G.
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