Immunoassay using an amplified cyclic detection system

Details Immunoassay using an amplified cyclic detection system Immunoassay using an amplified cyclic detection system

1981-10-01

1984-05-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 435 26, G01N 3354

Patent

active

044462310

ABSTRACT:
An immunoassay is disclosed where the label is an enzyme which converts a precursor into a cycling factor which in turn is interconverted in a cycling detection system. The detection system is amplified by the enzyme label constantly increasing the amount of cycling factors in the system. The system of NADP to NAD with NAD cycling to NADH and back is preferred.

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Anggard et al., "Prostaglandins: Enzymatic Analysis", Science 163, (1-1969), pp. 479-480.

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