Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-09-21
1990-03-27
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 56, 422 58, 422101, 422 61, 435 4, 435805, 436501, 436518, 436524, 436528, 436531, 436548, 436708, 436817, 436824, G01N 2178, G01N 3353, G01N 33543
Patent
active
049120344
ABSTRACT:
An apparatus is provided for use in an immunoassay for the detection of a target analyte in a liquid sample, comprising a porous reactive filter, having an upper surface and a lower surface, to which is bound an antibody specific for the target antigen; a holder affixed to the porous filter, wherein the upper surface and lower surface of the reactive filter are accessible for passage of liquids through the reactive filter; an absorbent container capable of being detachably attached to the holder; and an absorbent material contained within the container so as to contact the lower surface of the porous filter when the absorbent container is attached to the holder. A prefilter assembly can optionally be attached to the holder so that the prefilter contacts the upper surface of the reactive filter. The prefilter assembly, if present, and the absorbent container can readily be detached from the holder to avoid problems of back flow and to allow ease of storage of the disassembled holder and reactive filter for analysis and comparison with related assays, optionally a series of assays run at different times.
REFERENCES:
patent: 3723064 (1973-03-01), Liotta
patent: 3888629 (1975-06-01), Bagslawe
patent: 4246339 (1981-01-01), Cole et al.
patent: 4378428 (1983-03-01), Farina
patent: 4431545 (1984-02-01), Pall
patent: 4622207 (1986-11-01), Wang
patent: 4632901 (1986-12-01), Valkirs
French Larry D.
Kalra Krishan L.
Moon Patricia A.
Pawlak Katarzyna
Biogenex Laboratories
Hill Jr. Robert J.
Neeley Richard L.
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