Chemistry: physical processes – Physical processes – Crystallization
Patent
1976-10-07
1978-08-01
Serwin, R.E.
Chemistry: physical processes
Physical processes
Crystallization
23253R, 424 1, 424 15, 424 12, G01N 3316, G01N 2312
Patent
active
041040260
ABSTRACT:
In a method for effecting immunoassay of a multiplicity of samples, each possibly containing an antigen or an antibody to be assayed, wherein each sample is incubated with a solution containing a detectable antigen or antibody to form a multiplicity of mixtures, each mixture containing as components complexed antigen-antibody, non-complexed antigen and non-complexed antibody, separating at least one of the components of said mixture by adsorption and thereafter detecting the quantity of detectable antigen or antibody, in one of the non-adsorbed portions of the mixture,
The improvement which comprises continuously and sequentially separating at least one component intended to be separated from each of said multiplicity of mixtures by
Passing a first mixture from said multiplicity of mixtures over an adsorbent which adsorbs the components intended to be separated from said mixture,
Removing from said adsorbent the non-adsorbed portion of said mixture, and
Repetitively passing each next succeeding mixture from said multiplicity of mixtures over the same adsorbent without intermittent removal of the components of the mixture which had been adsorbed onto said adsorbent from the preceding mixtures, and removing from said adsorbent the non-adsorbed portion following each pass,
Wherein each sequential mixture is in contact with said adsorbent for a residence time which is sufficient to permit adsorption of the said component, but which is insufficient to effect significant disassociation of the prior adsorbed component, and which is insufficient to effect adsorption of additional components of said mixture onto the additional adsorption sites created on said adsorbent by the prior adsorption of the components from the preceding mixture. Apparatus is also provided for effecting said method.
REFERENCES:
patent: 3690833 (1972-09-01), Ferrari
patent: 3843326 (1974-10-01), Lichtenstenen
patent: 3896217 (1975-07-01), Johnson
patent: 4009005 (1977-02-01), Johnson
Brooker Gary
Price Michael G.
Terasaki Wesley L.
Serwin R.E.
University of Virginia
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