Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1981-01-12
1982-08-03
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435177, 435182, 435188, 435810, 23230B, 424 12, C12W 1104, G01W 3354
Patent
active
043428266
ABSTRACT:
An immunoassay method utilizes antigen tagged, enzyme encapsulating liposomes which are immunospecifically ruptured in the presence of cognate antibody and active complement. A homogeneous phase reaction occurs with the antibody and complement acting to release the enzyme if an immunospecific antigen-antibody complex is formed at the surface of the liposome. The positions of the antigen and antibody can be reversed.
REFERENCES:
patent: 3850578 (1974-11-01), McConnell
patent: 3887698 (1975-06-01), McConnell et al.
patent: 3937668 (1976-02-01), Zolle
patent: 4021364 (1977-05-01), Sperser et al.
patent: 4078052 (1978-03-01), Papahadjopoulos et al.
patent: 4193983 (1880-03-01), Ullman et al.
patent: 4235792 (1980-11-01), Hsia et al.
patent: 4235871 (1980-11-01), Papahadjopoulos et al.
patent: 4241046 (1980-12-01), Papahadjopoulos et al.
Wei, et al., J. Immunol Methods, vol. 9, (1975), pp. 165-170.
Rosenquist, et al., J. Immunol Methods, vol. 15, (1977), pp. 147-155.
Chan, et al., J. Immunol Methods, vol. 21, (1978), pp. 185-195.
Hagis, et al., Biochem. Biophys. Res. Comm., vol. 95, (1980), pp. 187-192.
Shiba, et al., "Thin-Layer Potentiometric Analysis of Lipid Antigen-Antibody Reaction by Tetrapontylammonium (TPA+) Ion Loaded Liposomes and TPA & Ion Selective Electrode", Anal Chem., vol. 52, No. 11, (1980), pp. 1610-1613.
Gregoriadis, et al., Liposomes in Biological Systems, 156, 158, 159 & 174-178.
Smolasky, et al., J. Immunol. Methods, vol. 15, (1977), pp. 255-265.
D'Osazlo et al., Anal. Chem., vol. 49, (1977), pp. 2083-2086.
Strejnn, et al., "Naturally Occurring Antibodies to Liposomes", J. Immunol., vol. 123, No. 1, (1979), pp. 370-378.
Uemura et al., "Active vs Passive Sensitization of Liposomes Toward Antibody and Complement by Dinitrophenylated Derivatives of Phosphatidylethanolamine", Biochem., vol. 11, No. 22, (1972), pp. 4085-4094.
Kataoka, et al., "Release of Macromolecular Markers (Enzymes) from Liposomes Treated with Antibody and Complement", Biochimica Biophysica ACTA, vol. 293, No. 2 (1973), pp. 158-179.
Rosenthal, et al., "Evaluation of Enzyme-Multiplied Immunoassay Technique (EMIT) for Determination of Serum Digoxin", Clin. Chem., vol. 22, No. 11, (1976), pp. 1899-1902.
Collaborative Research, Inc.
Wiseman Thomas G.
LandOfFree
Immunoassay products and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunoassay products and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay products and methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1468718