Immunoassay on a preblocked solid surface

Details Immunoassay on a preblocked solid surface Immunoassay on a preblocked solid surface

1991-09-30

1993-05-04

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 72, 435 792, 435 18, C12Q 170, C12Q 100

Patent

active

052081435

ABSTRACT:
A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

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Biochem. J. vol. 55, 1953, Morton, R. K., "Alkaline Phosphatase of Milk", pp. 795-800.

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