Immunoassay of PIVKA-II

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S007230, C435S007400, C435S007600, C435S007920, C435S013000, C435S452000, C435S337000, C435S338000, C435S962000, C436S518000, C436S534000, C436S548000, C530S384000, C530S388250, C530S388260, C530S389300

Reexamination Certificate

active

06893831

ABSTRACT:
The present invention provides an immunoassay for specifically measuring with high sensitivity PIVKA-II in serum or plasma through antigen-antibody reaction by adding an animal serum containing thrombin and/or an antibody reacting with human fibrin-like related substances to the reagents. The immunoassay of the invention comprises the steps of adding thrombin and/or an antibody reacting with human fibrin-like related substances to the reagents, and measuring PIVKA-II in serum or plasma.

REFERENCES:
patent: 4780410 (1988-10-01), Matsuda et al.
patent: 5516640 (1996-05-01), Watanabe et al.
patent: 5-249108 (1993-09-01), None
patent: 5-284994 (1993-11-01), None
patent: 7-313186 (1995-12-01), None
patent: 9-43237 (1997-02-01), None
patent: 9-249699 (1997-09-01), None
Lämmle et al., 1985. “Formation of the fibrin clot: the balance of procoagulant and inhibitory factors,” inClinics In Haematology. Coagulation Disorders.(Ruggeri, ed.) W.B. Saunders Company, London. pp. 281-285.*
Weir, 1978.Handbook of Experimental Immunology.Blackwell Scientific Publications, Oxford. p. 12. 13.

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