Immunoassay of phospholipid, such as phosphatidyl choline, in fl

Details Immunoassay of phospholipid, such as phosphatidyl choline, in fl Immunoassay of phospholipid, such as phosphatidyl choline, in fl

1982-09-29

1984-07-10

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

436 63, 436 71, 436543, 436547, 436811, 436815, G01N 3354, G01N 3392

Patent

active

044593627

ABSTRACT:
An immunologic assay for determining the presence of one or more phospholipids in a biological fluid. The method includes adding an ethanolic solution of diacylphosphatidylcholine or alkyltrimethylammonium halide and cholesterol to the biological fluid forming a macromolecular aggregate complex suspension. To the macromolecular aggregate complex solution is then added either an additional quantity of the biological fluid or a buffer reagent. The product of the reaction is then added to antibody molecules to the phospholipid and examined to determine the presence of the phospholipids.
The method is particularly useful in determining the presence of phosphatidylglycerol in a sample of amniotic fluid.

REFERENCES:
patent: 4233032 (1980-11-01), Statland
patent: 4257771 (1981-03-01), Yee
patent: 4290772 (1981-09-01), Frey
patent: 4292041 (1981-09-01), Fullerton
patent: 4370311 (1983-01-01), Ilekis
Chemical Abstracts, 92:142867m, (1980).
Chemical Abstracts, 93:128400t, 112185r and 112186s, (1980).
I. Inoue et al., Biochimica et Biophysica Acta, vol. 144, No. 2, issued Oct. 2, 1967, "Immunochemical Studies of Phospholipids. III Production of Antibody to Cardiolipin", pp. 409-414.
Gluck, L., et al., Am. J. Obstet. Gynecol., 109:3, 440-445, 1971.
Hallman, M. and Gluck, L., J. Lipid Res., 17, 257-262, 1976.
Hallman, M., et al., Am. J. Obstet. Gynecol., 125, 613-617, 1976.
Cunningham, M D., et al., Am. J. Obstet. Gynecol., 131, 719-724, 1978.
Freer, D. E., et al., Clin. Chem., 25, 960-968, 1978.
Bustos, R., et al., Am. J. Obstet. Gynecol., 133, 399-903, 1979.
Kulovich, M. V., et al., Am. J. Obstet. Gynecol., 135, 57-63, 1979.
Kulovich, M. V. and Gluch, L., Am. J. Obstet. Gynecol., 135, 64-70, 1979.
Tsai, M. Y., and Marchall, J. G., Clin. Chem., 25, 682-685, 1979.
Bent, A. E., et al., Am. J. Obstet. Gynecol., 139, 259-263, 1981.
Tsao, F. H. C., and Zachman, R. D., Clin. Chem. Acta, 118, 109-120, 1982.
Freer, D. E., and Statland, B. E., Clin. Chem., 27, 1629-1641, 1981.
Bligh, E. G., and Dyer, W. J., Can. J. Biochem. Physiol., 37, 911-917, 1959.
Wells, M. A., and Hanahan, D. J., Biochemistry, 8, 414-424, 1969.
Dittmer, J. C. and Wells, M. A., Methods in Enzymology, XIV Lipids, J. M. Lowenstein, Ed., Academic Press, N.Y., 1969, pp. 482-530.
Inoue, K. and Nojima, S., Biochem. Biophys. Acta., 144, 409-414, 1967.
Harris A., Rosenberg, A. A. and Riedel, L. M., J. Ven. Dis. Inform., 27, 169-174, Jul. 1946.
Garvey, J. S., Cremer, N. E. and Sussdorf, D. H., Methods in Immunology, 3rd Ed., W. A. Benjamin, Inc., Mass., 1977, pp. 218-219.
Maltaner, E. and Maltaner, F., Immunol., 51, 195-214, 1945.
Gotelli, G. R., et al., Clin. Chem., 24:7, 1144-1146, 1978.
Gluck, L., Clin. Chem. 23:1107, 1977.
Ip, M. P. C. et al., Clin. Chem., 23:35, 1977.
Mackenna, J., et al., Obstet. & Gynecol., 57:4, 493-495, 1981.
Cavalieri, R. et al., Amer. J. Obstet. Gynecol., 141:6, 652-656, 1981.
Freer, D. E. et al., Clin. Chem., 25:6, 960-968, 1979.
Jangalwala, F. B. et al., Biochem. J., 155, 55-60, 1976.
Anaokar, S. et al., Clin. Chem., 25:1, 103-107, 1978.
Nelson, G. H., Am. J. Obstet. Gynecol., 115:7, 933-941, 1973.
Jimenez, J. M. et al., Gynecol. Invest., 5:245-251, 1974.
Duck-Chong, C. G. et al., Clin. Chem., 26/6, 766-769, 1980.
Tiwary, C. M., and Goldkrand, J. W., Obstet. & Gynecol., 48:2, 191-194, 1976.
Clements, J. A. et al., N. Eng. J. Med., 286-20, 1077-1081, 1972.
Shinitzky, M. et al., Br. J. Obstet. Gynecol., 83, 838-844, 1976.
Sbarra, A. J. et al., Obstet. Gynecol., 48:5, 613-615, 1976.
Sing, E. J., Am. J. Obstet. Gynecol., 136:2, 228-229, 1980.

Affiliated with

Also associated with

Rating

Immunoassay of phospholipid, such as phosphatidyl choline, in fl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay of phospholipid, such as phosphatidyl choline, in fl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay of phospholipid, such as phosphatidyl choline, in fl will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1576734