Immunoassay of glycosylated proteins employing antibody directed

Details Immunoassay of glycosylated proteins employing antibody directed Immunoassay of glycosylated proteins employing antibody directed

1993-11-12

1996-01-16

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

Monoclonal antibody

435 71, 435 79, 435 14, 43524027, 435 7021, 436536, 5303875, 5303879, 53038825, 5303893, G01N 3353, G01N 33542

Patent

active

054847354

ABSTRACT:
An immunoassay for a protein that is non-enzymatically glycosylated on the alpha amino group of its N-terminal amino acid, the immunoassay comprising: providing a sample containing the glycosylated protein; reacting the glycosylated protein with a reducing agent so that the sugar residue on the N-terminal amino acid is reduced; contacting the reduced glycosylated protein with an antibody directed to Glc-ol-X which is prepared by immunizing an animal with an immunogen of the formula (Glc-ol-X-L).sub.n -carrier; and detecting or quantitating the reduced glycosylated protein bound to the antibody. In the formula (Glc-ol-X-L).sub.n -carrier: X is the N-terminal amino acid of the glycosylated protein, except that X cannot be lysine; L is a bond or a linker group; Glc-ol is the reduced form of the sugar attached to X on the glycosylated protein, and Glc-ol is attached to the alpha amino group of X; the carrier is an immunogenic compound other than the glycosylated protein; and n is from 1 to the number of available coupling sites on the carrier. Also, the antibody, the immunogen, and methods of making them. Finally, a kit for quantitating a protein glycosylated on its N-terminal amino acid.

REFERENCES:
patent: 3941763 (1976-03-01), Sarantakis
patent: 4200435 (1980-04-01), Stroupe et al.
patent: 4247533 (1981-01-01), Cerami et al.
patent: 4255385 (1981-03-01), Stroupe et al.
patent: 4268270 (1981-05-01), Gabbay et al.
patent: 4269605 (1981-05-01), Dean et al.
patent: 4349352 (1982-09-01), Manning et al.
patent: 4371374 (1983-02-01), Cerami et al.
patent: 4372747 (1983-02-01), Gabbay et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4399227 (1983-08-01), Niederau et al.
patent: 4423034 (1983-12-01), Nakagawa et al.
patent: 4438204 (1984-03-01), Deeg et al.
patent: 4478744 (1984-10-01), Mezei et al.
patent: 4629022 (1986-12-01), Dean
patent: 4629692 (1986-12-01), Dean
patent: 4636463 (1987-01-01), Altman et al.
patent: 4647654 (1987-03-01), Knowles et al.
patent: 4649122 (1987-03-01), Lee
patent: 4658022 (1987-04-01), Knowles et al.
patent: 4727036 (1988-02-01), Knowles et al.
patent: 4778752 (1988-10-01), Curtiss et al.
patent: 4797473 (1989-01-01), Tarsio et al.
patent: 4806468 (1989-02-01), Wagner et al.
patent: 4810391 (1989-03-01), Bruegger
patent: 4835097 (1989-05-01), Saunders
patent: 4837170 (1989-06-01), Ohe et al.
patent: 4861728 (1989-08-01), Wagner
patent: 4868130 (1989-09-01), Hargreaves
patent: 4876188 (1989-10-01), Smith et al.
patent: 4879036 (1989-11-01), Takahashi et al;.
patent: 4956301 (1990-09-01), Ismail et al.
patent: 5071767 (1991-12-01), Portenhauser et al.
patent: 5116762 (1992-05-01), Vogt et al.
patent: 5149633 (1992-09-01), Vogt et al.
patent: 5206144 (1993-04-01), Zeuthen et al.
Abraham et al., J. Lab. Clin. Med., 102, 187 (1983).
Avrameas and Ternynck, Immunochemistry, 6, 53 (1969).
Bannon et al., Clinical Chemistry, 30, 485 (1984).
Berzofsky et al., Biochemistry, 15, 2113 (1976).
Broughton and Strong, Clinical Chemistry, 22, 726 (1976).
Brownles et al., Diabetes, 29, 1044 (1980).
Brownlee et al., Ann. Int. Medicine, 101, 527 (1984).
Bunn, Diabetes, 30, 613 (1981).
Bunn, Amer. J. Med., 70, 325 (1981).
Cole et al., Metabolism, 27, 289 (1978).
Cully et al., Recent Progress in Clinical Chemistry (scientific presentation Sep. 1992).
Curtiss and Witzum, Diabetes, 34, 452 (1985).
Davis et al., Fed. Proc., 42, 1872 (1983).
Davis et al., Biochemistry International, 10, 395 (1985).
Day et al., J. Biol. Chem., 254, 595 (1979).
Dische et al., Arch. Biochem. Biophys., 2, 169 (1949).
Ehrlich et al., J. Immunology, 131, 1906 (1983).
Fairbanks and Zimmerman, Mayo Clin. Proc., 58, 770 (1983).
Friedman et al., Int. J. Peptide Protein Res., 6, 183 (1974).
Friquet et al., Molecular Immunology, 21, 673 (1984).
Fujiwara et al., abstract of presentation at 31st Immunometric Assay Symposium, Nov. 14, 1993.
Furth, New Scientist, 3, 58 (1988).
Furth, Analytical Biochemistry, 175, 374 (1988).
Garlick and Mazer, J. Biol. Chem., 258, 6142 (1983).
Go et al., Clin. Chim. Acta., 163, 63 (1987).
Goldstein, New Engl. J. Med., 384 (1984).
Gould et al., Ann. Clin. Biochem., 21, 16 (1984).
Hayashi and Makino, Clin. Chim. Acta, 149, 13 (1985).
Herold et al., Ann. Clin. Lab. Science, 13, 482 (1983).
Hjerten and Yang, J. Chromatography, 316, 301 (1984).
Javid et al., Brit. J. Haemotology, 38, 329 (1978).
Jovanovic and Peterson, Am J. Med., 70, 331 (1981).
Kemp et al., J. Pediatrics, 105, 394 (1984).
Kennedy and Merimee, Ann. Int. Med., 95, 56 (1981).
Kohler and Milstein, Nature, 256, 495 (1975).
Lane et al., J. Immun. Methods, 92, 261 (1986).
Little et al., Clin. Chem., 29, 1080 (1983).
Little et al., Clin. Chem., 29, 1113 (1983).
Lowrey and Soeldner, Analytical Biochemistry, 154, 424 (1986).
Makhlouf et al., Carbohydrate Research, 132, 93 (1984).
McDonald et al., J. Biol. Chem., 253, 2327 (1978).
Means and Feeney, Biochemistry, 7, 2192 (1968).
Merrifield J. Am. Chem. Soc., 85, 2149 (1963).
Miedema and Casparie, Ann. Clin. Biochem., 21, 2 (1984).
Moore et al., Ann. Clin. Biochem., 23, 85 (1986).
Moyle et al., J. Immunology, 20, 439 (1983).
Nakayama et al., Clin. Chim. Acta, 158, 293 (1986).
Nathan et al., New Engl. J. Med., 310, 341 (1984).
Nerenberg et al., J. Immun. Methods, 24, 19 (1978).
Ney et al., Analytical Biochemistry, 118, 294 (1981).
Olufemi et al., Clin. Chim. Acta, 163, 125 (1987).
Peacock, J. Clin. Pathol., 37, 841 (1984).
Playfair et al., Br. Med. Bull., 30, 24 (1974).
Reif, Immunochemistry, 6, 723 (1969).
Rendell et al., Clin. Chem., 31, 229 (1985).
Rucklidge et al., Biochemica et Biophysica Acta, 747, 165-170 (1983).
Shapiro et al., J. Biol. Chem., 255, 3120 (1980).
Smith et al., Anal. Biochem., 150, 76 (1985).
Staros et al., Anal. Biochem., 156, 220 (1986).
Svendsen et al., Diabetologia, 21, 549 (1981).
Waddell and Hill, J. Lab. Clin. Med., 48, 311 (1956).
Walton et al., Carbohydrate Research, 153, 285-293 (1986).
Walton and McPherson, Carbohydrate Research, 153, 285 (1986).
Weinryb et al., Drug Metab. Rev., 10, 271 (1979).
Witzum et al., Proc. Natl. Acad. Sci., 80, 2757 (1983).
Wong et al., Anal. Biochem., 139, 58 (1984).
Yue et al., Diabetologia, 24, 377 (1983).
Yue et al., Diabetes, 29, 296 (1980).
Yue et al., Diabetes, 31, 701 (1982).
Curtiss et al, J. Clin. Invest., vol. 72, 1427 (1983).

Affiliated with

Also associated with

Rating

Immunoassay of glycosylated proteins employing antibody directed does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay of glycosylated proteins employing antibody directed, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay of glycosylated proteins employing antibody directed will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-309029