Immunoassay of apolipoprotein-B of low density lipoproteins in p

Details Immunoassay of apolipoprotein-B of low density lipoproteins in p Immunoassay of apolipoprotein-B of low density lipoproteins in p

1986-02-14

1991-11-12

Wax, Robert A.

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

Through a gel

436 71, 436316, 436811, 435 71, 2041828, 204299R, G01N 33561, G01N 3353, C12Q 100, C25B 100

Patent

active

050647695

ABSTRACT:
Method of assaying the apolipoprotein B (apo B) of low density lipoproteins (LDL) in serum enabling the performance successively, in a single operation, of separation of the LDLs from other lipoproteins containing apo-B by electrophoresis in a polyacrylamide gel having a controlled degree of cross-linking and assay of the apo-B of the LDLs thus separated by electro-immunodiffusion in an agarose gel containing anti-apo B. Samples are placed in wells formed in the acrylamide gel, and the height of the precipitation arcs in the agarose gel is measured.

REFERENCES:
patent: 3930983 (1975-01-01), Sieber
Kistner, G. M. et al., Atherosclerosis 38 (1981) pp. 57-61.
Krauss, R. M. et al., J. Lipid Res. 23 (1982) pp. 97-104.
Sniderman, A. et al., Proc. Natl. Acad. Sci. U.S.A. 77 (1980) pp. 604-608.
Kwapinski, G., The Methodology of Investigative and Clinical Immunology, R. E. Krieger Pub. Co., Fla., 1982, p. 56.
Chemical Abstracts, vol. 93, No. 1, Issued Jul. 1, 1980, p. 317, #3248t, Heuck, C. C., "Methodological Aspects . . . Immonoeketrophoresis".

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