Immunoassay of antigens

Details Immunoassay of antigens Immunoassay of antigens

1983-09-26

1987-04-21

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving antibody fragments

436513, 436518, 436531, 436548, 436800, 436808, 436819, 436822, 436828, G01N 33563, G01N 3353, G01N 33577, B65D 6900

Patent

active

046596780

ABSTRACT:
A method of immunoassay of an antigen in a liquid sample wherein a complex is formed between antigen contained in the said sample and two or more antibody reagents, and the said complex is bound to a solid support by non-covalent bonding as defined herein; and the amount of complex becoming bound to the support is determined; the process employing at least one monoclonal antibody reagent. Labelling methods including radio-active, fluorimetric and enzyme labelling may be used to effect determination of the binding of the complex to the solid support. The solid support may take the form of particles, beads, wall-coatings on the reaction vessel or an insert of large surface area. The method is particularly applicable to the assay of TSH, CEA, HCG, alphafeto protein, immunoglobulins, viruses, allergens, bacteria, toxins, drugs and vitamins. Use of monoclonal reagents improves the specificity of the process, and also decreases non-specific binding.

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