Immunoassay methods employing patterns for the detection of solu

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436524, 436528, 436537, 436541, 436542, 436800, 436804, 436807, 436808, 436809, 436823, G01N 3352, G01N 3358

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active

044878397

ABSTRACT:
Methods for determining the presence of antigens or antibodies in an aqueous sample or presence of antigens on the surface of cells. A preferred embodiment employs fluorescent antigens which compete with the sample antigens for antibody binding sites. The antibodies are deposited on a support surface means in alternating patterns. The surface means and fluorescence detector are translocated with respect to each other and a signal generated by the detection of the repeating pattern of fluorescence. The signal is analyzed by means of a gated integrator responsive to a gate track control means also located on the surface means. Immunoassay methods having increased sensitivity are thereby obtained.

REFERENCES:
patent: 3941876 (1976-03-01), Marinkovich
patent: 4011308 (1977-03-01), Giaever
patent: 4031197 (1977-06-01), Marinkovich
Walden, Diss. Abstracts B, vol. 41 (1980), pp. 561-562.
Deeloer et al., Chem. Abstracts, vol. 90 (1979) #68828q.

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