Immunoassay methods and reagents and methods for producing the l

Details Immunoassay methods and reagents and methods for producing the l Immunoassay methods and reagents and methods for producing the l

1988-05-06

1992-02-18

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436546, 436800, 436817, G01N 33543

Patent

active

050894234

ABSTRACT:
Immunoassay and methods for using fluorescent chelates of lanthanide metal ions in conjunction with immunoreactive bodies to permit fluorescent assay in liquid or dry samples. The assay reagent comprises a residue of an immunoreactive body linked to a residue of a protein or polypeptide. The protein or polypeptide is labeled by substitution with a ligand forming a fluorescent chelate with lanthanide metal ion such as europium. The reagent when used in an immunoassay, binds to the immobilized immunoreactive body and excess is washed away. The sample can be dried for later analysis by a suitable fluoremeter.

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Uotila et al., Chemical Abstracts, vol. 94;190054g (1981).
The Wako Pure Chemical Industries, Ltd., Chemical Abstracts, vol. 101:104944f (1984)

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