Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-07-15
1988-09-27
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 21, 435 28, 436530, 436810, G01N 3353, G01N 33544
Patent
active
047741775
ABSTRACT:
A method for detecting the presence of antibody or antigen in a liquid sample suspected of containing the antibody or antigen. To a test panel comprising a nitrocellulose carrier having stably bonded thereto a microsized spot consisting of either a primary ligand for that antibody or an antibody for that antigen, is added sufficient liquid containing non-specific globulin for the material of the spot, to block adjacent binding sites. Then a small liquid drop of the sample suspected of containing the antibody or antigen is added. After incubation of the panel, in tests for detecting an antibody, a drop of liquid containing labeled antiglobulin, which is an anti-immunoglobulin for that antibody, is added. In tests for detecting an antigen, the specific antibody-enzyme conjugate is added. After further incubation wash solution is added to the spot, and the test panel is assayed for presence of the label.
REFERENCES:
patent: 4446232 (1981-10-01), Liotta
patent: 4452901 (1984-06-01), Gordon et al.
Hawkes et al., Analytical Biochemistry, vol. 119 (1982), pp. 142-147.
W. Neal Burnetti: "Western Blotting" etc.; 112 Analytical Biochemistry, 195-203 (1981), pp. 195-203.
Christiane Rordorf et al., "A Multidot Immunobinding Assay (etc.), 59 Journal of Immunological Methods 105-112 (1983).
McDougall et al., "Immunidot Assay . . . etc." 63 Journal of Immunological Methods 281-290 (1983).
Jan Marks, "Minimizing the Problems of Collecting Blood", Veterinary Medicine, Dec. 1984, pp. 1497-1500.
Rosen Sam
Vet-Test Partners
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