Immunoassay method for detecting viral antibodies in whole blood

Details Immunoassay method for detecting viral antibodies in whole blood Immunoassay method for detecting viral antibodies in whole blood

1979-10-19

1982-02-02

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 8, 424 12, 435 7, G01N 3356, G01N 3358

Patent

active

043139272

ABSTRACT:
An immunoassay method for the detection of an antibody (Ab.sub.1) to a viral antigen (Ag) wherein a whole human blood sample diluted with an isotonic aqueous solution is incubated with a solid-phase form of Ag whereby any Ab.sub.1 present in the sample becomes bound to solid-phase Ag, the resulting solid-phase Ag-Ab.sub.1 complexes are separated from the sample, a label-incorporated form of an antibody to Ab.sub.1 (Ab.sub.2 *) is contacted with the separated, solid-phase Ag-Ab.sub.1 complexes, the resulting solid-phase Ag-Ab.sub.1 -Ab.sub.2 * complexes are separated from excess Ab.sub.2 *, and the amount of the label in the separated, solid-phase Ag-Ab.sub.1 Ab.sub.2 * complexes is measured as a function of the presence of Ab.sub.1 in the sample. Preferably the whole blood sample is diluted 1:20 by volume with an isotonic aqueous buffer solution. The method is particularly useful for the detection of cytomegalovirus antibody or Rubella antibody.

REFERENCES:
patent: 4178360 (1979-12-01), Cleeland et al.
patent: 4185084 (1980-01-01), Mochida et al.

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