Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-11-07
1991-12-17
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436527, 436824, 436825, G01N 33552
Patent
active
050734857
ABSTRACT:
An immunoassay method which measures test samples at high concentration without dilution is disclosed. Said immunoassay comprises the steps of: (1) reacting a material (A) to be measured, an immobilized component (B) which binds specifically to the material (A), and a labelled component selected from the group consisting of (i) a labelled component (C) which binds specifically to the material (A) and (ii) a labelled component (A) thereby obtaining an immuno-complex and (2) detecting or measuring the labelling fragment, wherein the reaction of the material (A) with the immobilized (B) or the reaction of the material (A) with the immobilized (B) and the labelled component is carried out at a pH of 2-4.5.
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Clin. Chem. 28/10,2033(1982) .beta..sub.2 -Microglobulin Determined by Radioimmunoassay with Monoclonal Antibody, R. Swanson, R. Tracy, J. Katzmann, et al.
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Clin. Chem. 29/7,1437(1983) Enzyme Immunoassay of Thyroxin-Binding Globulin in Dried Blood Samples on Filter Paper, N. Hata, M. Ito, H. Mizuta, et al.
Amano Yoshiyuki
Kikutake Jun-ichiro
Sugiura Masakazu
Nucker Christine
Sanyo Chemical Industries Ltd.
Stucker Jeffrey
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