Immunoassay method and kit to be used therein

Details Immunoassay method and kit to be used therein Immunoassay method and kit to be used therein

2003-12-24

2009-11-03

Lam, Ann Y. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007100, C435S007920, C435S007930, C435S007950, C435S021000, C435S070210, C435S452000, C435S174000, C435S177000, C435S338000, C435S962000, C435S975000, C436S518000, C436S523000, C436S528000, C436S534000, C436S548000, C436S811000, C530S388260, C530S391100

Reexamination Certificate

active

07611855

ABSTRACT:
Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.

REFERENCES:
patent: 4745072 (1988-05-01), Ekins et al.
patent: 4769320 (1988-09-01), Furie et al.
patent: 5037764 (1991-08-01), Wilk et al.
patent: 5382530 (1995-01-01), Romelli et al.
patent: 6010866 (2000-01-01), Ollington et al.
patent: 6190873 (2001-02-01), Davalian et al.
patent: 6248544 (2001-06-01), Halleen et al.
patent: 6737278 (2004-05-01), Carlsson et al.
patent: 7074903 (2006-07-01), Ohashi et al.
patent: 1 359 161 (2003-05-01), None
patent: 60-501674 (1985-10-01), None
patent: 63-30766 (1988-02-01), None
patent: 6-289020 (1994-10-01), None
patent: 10-221341 (1998-08-01), None
patent: 10-337198 (1998-12-01), None
patent: 2001-281222 (2001-10-01), None
patent: 2002-31640 (2002-01-01), None
patent: WO 85/00226 (1985-01-01), None
patent: WO 01/25791 (2001-04-01), None
Janckila et al., 1997. Characterization of monoclonal antibodies specific to tartrate-resistant acid phosphatase. Hybridoma 16(2): 175-182.
J.M. Halleen, et al.; “Tartrate-Resistant Acid Phosphatase 5b: A Novel Serum Marker of Bone Resorption;”Journal of Bone and Mineral Research; vol. 15; No. 7; Jul. 2000; Cover Sheet, Index and pp. 1337-1345 (12 Sheets.).
A.J. Janckila, et al.' “Tartrate-resistant Acid Phosphatase Isoform 5b as Serum Marker for Osteroclasitc Activity;”Clinical Chemistry; vol. 47; No. 1; Jan. 2001; Cover Sheet, Index and pp. 74-80 (11 Sheets.).
P.D. Delmas; “Standardization of Bone Marker Nomenclature;”Clinical Chemistry; vol. 47; No. 8; Aug. 2001; Cover page, index and p. 1497 (6 Sheets.).
“Nichidai-Ishi;” Vol. 49; No. 9; 1990; Cover Sheets and pp. 904-911 (6 Sheets.), Abstract only.
K.-H.W. Lau, et al.; “Characterization and Assay of Tartrate-Resistant Acid Phosphatase Activity in Serum: Potential Use to Assess Bone Resportion;”Clinical Chemistry; vol. 33; No. 4; Apr. 1987; Cover Sheet., index and pp. 458-462; (10 Sheets.).
M.E. Kraenzlin, et al; “Development of an Immunoassay for Human Serum Osteoclastic Tartrate-Resistant Acid Phosphatase;”Journal of Clinical Endrocrinology and Metabolism; vol. 71; No. 2; Aug. 1990; Cover Sheet; Index and pp. 442-451. (15 Sheets.).
S.L. Alatalo, et al; “Rapid Screening Method for Osteoclast Differentiation in Vitro That Measures Tartrate-resistant Acid Phosphatase 5b Activity Secreted into the Culture Medium;”Clinical Chemistry; vol. 46, No. 11; Nov. 2000; Cover Sheet, Index and pp. 1751-1754 (8 Sheets.).
J.M. Halleen, et al; “Characterization of Serum Tartrate-Resistant Acid Phosphatase and Development of a Direct Two-Site Immunoassay;”Journal of Bone and Mineral Research; vol. 13; No. 4; Apr. 1998; Cover page, Index and pp. 683-687. (9 Sheets.).
H. Bull, et al; “Reactivity and assay restriction profiles of monoclonal and polyclonal antibodies to acid phosphatases: a preliminary study;”Immunology Letters; vol. 70; No. 3; Dec. 1, 1999; Cover Sheet; Index and pp. 143-149 (9 Sheets.).
J.M. Halleen, et .; “Two-Site Immunoassays for Osteoclastic Tartrate-Resistant Acid Phosphatase Based on Characterization of Six Monoclonal Antibodies;”Journal of Bone and Mineral Research; vol. 14; No. 3; Mar. 1999; Cover Sheet, Index and pp. 464-469 (9 Sheets.).
Y.R. Nakasato, et al; “Clinical Significance of Immunoassays for Type-5 Tartrate-resistant Acid Phosphatase;”Clinical Chemistry; vol. 45; No. 12; Dec. 1999; Cover Sheet, Index and pp. 2150-2157 (13 Sheets.).
G. Köhler, et al.;“Continuous cultures of fused cells secreting antibody of predefined specificity;”Nature; vol. 256; Aug. 7, 1975; Cover Sheet and pp. 495-497 (4 Sheets.).
N. Ulloa et al; “Characterization of Monoclonal Antibodies Against Apolipoproteins A-I and A-II. Epitope Expression in LpA-I and LpA-I:LpA-II Particles”; Hybridoma; Liebert; New York, NY, US. vol. 18, No. 6; Dec. 1, 1999; pp. 513-520, XP009014610.
Supplementary European Search Report dated May 26, 2008.
Chinese Office Action dated May 25, 2007 and English Translation.

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