Immunoassay method and kit

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 791, 435 792, 435963, 435966, 435975, 436519, 436534, 436535, 436547, 436817, 436828, 530402, 530817, 427 2, G01N 33535, G01N 33554

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active

050631519

ABSTRACT:
A method of binding an antibody with protein A cells is provided which includes a sequence of incubation and dilution steps to produce a preselected amount and concentration of antibody with a preselected distribution of antibody among the protein A cells. In addition, a method is provided for preparing an antibody entrapped porous matrix and apparatus which includes a specific porous matrix with a preselected position of antibody bound bacterium cells therein along with means for drawing fluids through the porous medium and means for facilitating the deposition of fluids onto the surface of the porous matrix. The apparatus and method is useful for testing for the level of progesterone in animal body fluids, such as milk, plasma, serum, whole blood and saliva.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3770383 (1973-11-01), Price
patent: 3888629 (1975-06-01), Bagshawe
patent: 3995018 (1976-11-01), Sjoquist
patent: 4230664 (1980-10-01), Cais
patent: 4235601 (1980-11-01), Deutsch et al.
patent: 4279885 (1981-07-01), Reese et al.
patent: 4280816 (1981-07-01), Elahi
patent: 4292296 (1981-09-01), Parsons, Jr.
patent: 4376110 (1983-03-01), David et al.
patent: 4394444 (1983-07-01), Cameron et al.
patent: 4399229 (1983-08-01), Kelton et al.
patent: 4517288 (1985-05-01), Giegel et al.
patent: 4592996 (1986-06-01), Yamanishi et al.
Jungers et al., J. Lab. Clin. Medicine 98, 30-36 (1981).
"The Use of Protein A-Containing Staphylococcus Aureus as a Solid Phase Anti-IgG Reagent in Radioimmunoassays as Exemplified in the Quantitation of .alpha.-Fetoprotein in Normal Human Adult Serum", Johnson, S. and Kronvall, G., Eur. J. Immunmol. 4:29-33, 1974.
"Detection of Membrane-Associated Antigens on Lymphoid Cells by Antibody Coupled to Staphylococcal Protein A", Kearney et al., The Journal of Immunology, vol. 114, No. 4, Apr., 1975.
"Rapid Isolation of Antigens from Cells with a Staphylococcal Protein A-Antibody Adsorbent: Parameters of the Interaction of Antibody-Antigen Complexes with Protein A", Kessler, S. W., The Journal of Immunology, vol. 115, No. 6, Dec., 1975.
"Cell Membrane Antigen Isolation with the Staphylococcal Protein A-Antibody Adsorbent", Kessler, S. W., The Journal of Immunology, vol. 117, No. 5, Part 1, Nov., 1976.
"Use of Immunoglobulin-Loaded Protein A-Bearing Staphylococci as a Primary Solid Phase Immunoadsorbent in Radioimmunoassay", O'Keefe, E. O. and Bennett, V., The Journal of Biological Chemistry, vol. 255, No. 2, Jan., 1980.
"Dipstick Method for Human Chorionic Gonadotropin Suitable for Emergency Use on Whole Blood and Other Fluids", Norman, R. J., et al., The Lancet, Jan. 5, 1985.
"ImmunoConcentration.TM.--A New Format for Solid-Phase Immunoassays", Valkirs, G. E., and Barton, R., Clinical Chemistry, vol. 31, No. 9, 1985.
"You're Looking at a Revolutionary Application of Monoclonal Antibody Technology: ImmunoConcentration.TM.", anonymous undated brochure published by Hybritech, Inc., San Diego, California.

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