Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-06-15
1989-04-11
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 15, 435 18, 435 25, 435 26, 435177, 435182, 436520, 436522, 436821, G01N 3353
Patent
active
048206342
ABSTRACT:
Immunoassay which can be performed within a short period of time and a reagent composition for such immunoassay. The reagent composition contains complement and cells which contain quantitatively determinable substance therein. Antibody or antigen corresponding to antigen or antibody to be determined in a sample is bound on the outer surfaces of the cell membranes, which can be lysed by the complement upon activation thereof. In the immunoassay, a sample containing antigen or antibody to be determined is mixed with the reagent composition. The complement is activated by the resultant antigen-antibody complex. The cell membranes are lysed by the complex and the determinable substance in the cells is liberated from the cells. The amount of the liberated substance is determined.
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D'Orazio et al., "Ion Electrode Measurements of Complement and Antibody Levels . . . ", Analytical Chemistry, vol. 49, No. 13, Nov. 1977, pp. 2083-2086.
Chemical Abstracts 78: 120694m.
Tokyo Shibaura Denki Kabushiki Kaisha
Warden Robert J.
Wieder Stephen
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