Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1983-08-08
1988-10-18
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 18, 435 21, 435 7, 435 4, 435 26, 435184, C12Q 154, C12Q 134, C12Q 142, C12Q 132, C12Q 100, C12N 999, G01N 33535
Patent
active
047787554
ABSTRACT:
In the determination of the quantity of enzyme present as a result of an immunoassay procedure, the enzyme containing component is combined with a substrate which in the presence of the enzyme yields a photometrically detectable enzymatic reaction product and a non-interfering, enzymatically inhibiting second product. The enzymatic reaction is inhibited by the addition of an inhibiting quantity of the same compound as the second product after the enzymatic reaction has continued for a time sufficient to produce photometrically detectable first products in concentrations which are a function of the quantity of enzyme present. The enzyme reaction is inhibited before the enzymatic reacton has proceeded to the point where the quantity of photometrically detectable first compound is primarily a function of the amount of substrate. This method yields a more stable test medium than methods using other enzyme inhibitors.
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Brauner et al., J. Immunol. Methods, 42: 375-379 (1981).
Whitaker, Principles of Enzymology for the Food Sciences, Marcel Dekker, Inc., New York.
Cain Susan M.
Tsay Yuh-geng
Goldman Philip M.
Kepplinger Esther M.
Minnesota Mining and Manufacturing Company
Sell Donald M.
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