Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-05-14
1984-09-04
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 61, 435810, 436500, 436512, 436534, 436801, 436804, 436808, 436813, 436817, 436818, 436828, G01N 3354, G01N 3356, G01N 3358, G01N 3376
Patent
active
044697872
ABSTRACT:
A method and kit for determining the presence of a polyvalent ligand in an aqueous fluid. The method involves incubating the fluid with an immobilized antibody to the ligand to form an immobilized ligand-antibody complex, then incubating the immobilized ligand-antibody complex with a solution of a soluble complex of a second antibody (to the ligand) and a labeled binding protein, such as protein A. The labeled binding protein is specifically bound to the Fc portion of the second antibody. The unbound second antibody and labeled binding protein are washed from the immobilized complex, and the presence of bound labeled binding protein is determined as a measure of the concentration of the ligand in the aqueous fluid.
REFERENCES:
patent: 3791932 (1974-02-01), Schuurs
patent: 3876504 (1975-04-01), Koffler
patent: 3966898 (1976-06-01), Sjoquist
patent: 4001583 (1977-01-01), Barrett
patent: 4067959 (1978-01-01), Bolz
patent: 4169138 (1979-09-01), Jonsson
patent: 4200508 (1980-04-01), Hirai
patent: 4210418 (1980-07-01), Brown
patent: 4230685 (1980-10-01), Senyei
patent: 4230797 (1980-10-01), Boguslaski
patent: 4332783 (1982-06-01), Pernice
G. W. Notani et al., J. Histochem. & Cytochem., 27(11), 1438-1444 (1979).
A. H. W. M. Schuurs et al., Clinica Chimica Acta, 81, 1-40 (1977).
R. H. Jacobson et al., Amer. Assn. Veterinary Diagnosticians, 21st Annual Proceedings, 367-376 (1978).
E. O'Keefe et al., J. Biol. Chem., 255(2), 561-568 (1980).
H. A. Erlich et al., "Methods in Enzymology", vol. 68, pp. 443-453, Academic Press, 1979.
A. Voller et al., Bull. World Health Organ., vol. 53, 55-65 (1976).
J. J. Langone et al., J. Immunol. Methods, 18, 281-293 (1977).
J. J. Langone, J. Immunol. Methods, 24, 269-285 (1978).
J. J. Langone, J. Immunol. Methods, 34, 93.106 (1980).
E. Engvall, Scand. J. Immunol. vol. 8, Suppl. 7, 25-31 (1978).
Chapman Dana A.
St. Denis John N.
Woods James W.
Mallinckrodt Inc.
Marantz Sidney
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