Immunoassay for vitamin K-dependent bone protein

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody

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424 85, 435 7, 436545, 436546, 436547, 436800, 436804, 436811, G01N 3356, G01N 3358, G01N 3360

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active

044105061

ABSTRACT:
Immunoassay and compositions therefore for detection of human vitamin K-dependent bone protein. Heterologous labeled vitamin K-dependent protein or antigenic fragment thereof is employed with antibodies to the protein in an assay for the protein in various physiological fluids for bone extracts. The assay may be used in the diagnosis of bone diseases, by itself or in conjunction with an alkaline phosphatase assay.

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Hauschka et al., Proc. Natl. Acad. Sci. USA, 72:3925-3929 (1975).
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Price et al., Proc. Natl. Acad. Sci. USA, 77:2234-2238 (1980).
Poser et al., J. Biol. Chem., 255: 8685-8691 (1980).
Price et al., J. Biol. Chem., 256: 3781-3784 (1981).
Price et al., Biochem. Biophys. Res. Comm., 99: 928-935 (1981).

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