Immunoassay for the detection of serum analytes using pH depende

Details Immunoassay for the detection of serum analytes using pH depende Immunoassay for the detection of serum analytes using pH depende

1985-10-23

1987-10-27

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 4, 435 7, 435810, 436506, 436518, 436528, 436808, 436809, 436811, 436825, 436826, C12Q 170, G01N 3353, G01N 33564

Patent

active

047030011

ABSTRACT:
The performance of immunoassays for the analysis of serum analytes can be significantly improved by the pretreatment of the sample. Analyte in serum samples is often complexed with serum antibody. Such analyte-antibody complexes can mask the analyte and interfere with analyte specific binding steps of many immunoassays. The serum pretreatment method employs pH dependent chaotropes to dissociate the analyte-antibody complexes in the serum. At low pH, the complexes become dissociated and the antibody becomes denatured. After the dissociation and denaturation of the serum antibody, the serum sample and the chaotrope, contained therein, are then neutralized. Since the serum antibody has been denatured, it does not re-associate with the analyte upon neutralization. After the neutralization step, serum sample can then be analyzed by an immunoassay, without interference from serum antibody. A serum pretreatment kit is essential to the employment of the serum pretreatment method.

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von Schenck et al., Scano. J. Clin. Lab. Invest. 43(1983), 527-31.

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