Immunoassay for the detection of ligands

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 5, 435 6, 435 79, 435 723, 435 792, 435 793, 436518, G01N 3353

Patent

active

058957502

ABSTRACT:
A biotin-antibiotin immunoassay comprises coating a solid phase with a ligand-specific binding material, reacting the solid phase with a test sample, reacting the solid phase with the biotin-labeled form of the ligand-specific binding material and antibiotin labeled with a suitable marker. The marker is then measured to detect the amount of ligand present in the test sample.

REFERENCES:
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4535057 (1985-08-01), Dreesman et al.
patent: 4687732 (1987-08-01), Ward et al.
patent: 4945042 (1990-07-01), Geiger et al.
Brigati, et al., Biological Abstracts 76:5025 (Abstract No. 46212) (1983).
J.L. Guesdon, et al., J. Histochem. & Cytochem. 27(8):1131-1139 (1979).
M. Wilchek and E.A. Bayer, Immunology Today 5(2) 39-43 (Mar., 1984).
D.J. Brigati et al., Virology 126:32-50 (1983).
E.A.Bayer and M. Wilchek, Methods and Biochemical Analysis 26:1-45 (1980).
P.R. Langer-Safer et al., Proc. Natl. Acad. Sci. 79: 4381-4385 (1982).
I.K. Mushahwar et al. J Virol. Methods 16:45-54 (1987).
P. Vincent, et al., Journal of Immunological Methods 165 (1993) 177-182.
Wood et al. J. Histochem. Cytochem., 29(10):1196-1204 (1981).
Banerjee et al, J. Clin. Pathol., 37: 223-225 (1984).
Federation Proceedings, 46, Abstract 4911, p. 1159 (1987) Mock.
Langer P. et al., An Immunological Method of Gene Mapping by In Situ Hybridization (undated).
Langer P. et al., Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes, in Proc. Natl. Acad Sci. U.S.A vol. 78. No. 11 pp. 6633-6637, Nov. 1981.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Immunoassay for the detection of ligands does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immunoassay for the detection of ligands, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoassay for the detection of ligands will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2247599

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.