Immunoassay for the detection of collagen I

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 72, 435 793, 435810, G01N 3353

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active

058210652

ABSTRACT:
A method for determining collagen degradation products in a body fluid sample using a non-competitive or competitive immunoassay is disclosed, the method comprising incubating the body fluid sample with an amount of antibody which specifically reacts with the amino acid sequence of SEQ ID NO. 2 of collagen l or a fragment thereof, and determining the collagen degradation products bound to the amount of the antibody. A kit for such a method is also disclosed.

REFERENCES:
Burgess et al (J. Cell Bio, 111: 2129-2138, (1990).
Tao et al, 1989, (J. Immunol, 143: 2595-2601, 1989).
Lazar et al, (Mol & Cel Bio, 1988, 8: 1247-1252).
Herbert et al, Dictionary of Immunology, 4th Ed, Academic Press, London, pp. 3 & 147, 1995.
Bonde et al. Clin Chem. 40 (11) 2022, 2025, Nov. 1994.
Harlow & Lane Cold Spr Harbor Laboratory Press Ch. 14, 1988.

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