Immunoassay for peptide and protein oligomers

Details Immunoassay for peptide and protein oligomers Immunoassay for peptide and protein oligomers

1984-09-24

1986-11-18

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 436518, 436548, 436804, 436815, 935110, G01N 33534, G01N 33535, G01N 33543

Patent

active

046236210

ABSTRACT:
A convenient immunoassay for detecting the presence of oligomeric forms of peptides and proteins is described. The assay employs the sandwich technique with a monoclonal antibody selective for a single epitope on the peptide or protein bound to a solid phase and the same monoclonal antibody labeled with a detectable label in the solution phase.

REFERENCES:
patent: 4376110 (1983-03-01), David
patent: 4514507 (1985-04-01), Secher
Secher, D. S. et al., Nature, 285, 446-450 (1980).
"Principles of Biochemistry" by A. White et al., Fourth Edition, pp. 147-148, McGraw-Hill, New York 1968.
Staehelin, T. et al., J. Biol. Chem., 256(18), 9750-9754 (1981).
Staehelin, T. et al., Proc. Natl. Acad. Sci. USA, 78 (3), 1848-1852 (1981).

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