Immunoassay for opiate alkaloids and their metabolites; tracers,

Details Immunoassay for opiate alkaloids and their metabolites; tracers, Immunoassay for opiate alkaloids and their metabolites; tracers,

1986-07-14

1990-07-03

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436546, 436547, 436800, 436805, 436825, 436826, 546 44, 546 45, 546 46, 424 88, 530387, 530807, G01N 33542

Patent

active

049392646

ABSTRACT:
The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

REFERENCES:
patent: 4064228 (1977-12-01), Gross
patent: 4160016 (1979-07-01), Ullman
patent: 4213904 (1980-07-01), Haugland
patent: 4420568 (1983-12-01), Wang
patent: 4476229 (1984-10-01), Fino et al.
patent: 4668640 (1987-05-01), Wang
Gross-I: Immunochemistry 11, 453-456 (1974).

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