Immunoassay for lipoprotein(a)

Details Immunoassay for lipoprotein(a) Immunoassay for lipoprotein(a)

1988-02-29

1990-07-31

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436 63, 436 71, 435 11, G01N 3392

Patent

active

049450409

ABSTRACT:
The present invention relates to a method for the detection and quantification of lipoprotein(a) present in a fluid sample which method is not sensitive to the presence of plasminogen and which comprises contacting the fluid sample with anti-apo(a) antibody and forming a first immobilized complex of lipoprotein(a)/anti-apo(a) first antibody, contacting said first complex with anti-apo B second antibody and thereafter quantitating Lp(a) based on the amount of bound anti-apo B antibody. Immobilized rabbit anti-human apo(a) antibody can be employed as the first, capture, antibody and goat-anti-human apo B antibody as the second antibody. The amount of anti-apo B antibody bound is quantitated through binding of a third antibody/enzyme conjugate, e.g., a rabbit anti-goat antibody/alkaline phosphatase conjugate, followed by reaction with a suitable enzyme substrate such as paranitrophenyl phosphate.

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