Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-11-09
1997-11-25
Feisee, Lila
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435975, 436 63, 436808, 436826, G01N 3353, G01N 3348
Patent
active
056911505
ABSTRACT:
Described are immunoassay of insulin-like growth factors in a biological sample, which comprises prior to the immunoassay, treating the biological sample with an acid solution to liberate the insulin-like growth factors from insulin-like growth factor binding proteins and then neutralizing the so-treated biological sample with a neutralizing buffer containing a recombination inhibitor; and immunoassay kit for insulin-like growth factors.
REFERENCES:
Blum et al, "A specific radioimmunoassay for insulin-like growth factor II: the interference of IGF binding proteins can be blocked by excess IGF-I", Acta Enocrinol., vol. 118, pp. 374-380 1988.
Analytical Biochemistry, vol. 191, No. 1, pp. 75-77, Nov. 15, 1990, Thomas A. Bicsak, et al., "Insulin-Like Growth Factor Binding Protein Measurement: Sodium Dodecyl Sulfate-Stable Complexes with Insulin-Like Growth Factor in Serum Prevent Accurate Assessment of Total Binding Protein Content by Ligand Blotting".
Domestic Animal Endocrinology, vol. 8, No. 3, pp. 393-405, Jul. 1991, K. Plaut, et al., "Evaluation of Interference by Insulin-Like Growth Factor I (IGF-I) Binding Proteins in a Radioimmunoassay for IGF-I in Serum From Dairy Cows".
The Journal of Biological Chemistry, vol. 269, No. 32, pp. 20388-20393, Aug. 12, 1994, T. Arai, et al., "Heparin, Heparan Sulfate, and Dermatan Sulfate Regulate Formation of the Insulin-Like Growth Factor-I and Insulin-Like Growth Factor-Binding Protein Complexes".
Morris, J.C. et al., "Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods." Mayo Clin. Proc., 63:707-717 (1988).
Journal of Clinical Endocrinology and Metabolism, vol. 54, No. 5, pp. 1084-1086, May 1982, R. Furlanetto, "Pitfalls in the Somatomedin-C Radioimmunoassay".
Journal of Clinical Endocrinology and Metabolism, vol. 56, No. 2, pp. 384-389, Feb. 1983, D. Clemmons, et al., "Liberation of Immunoreactive Somatomedin-C From Its Binding Proteins by Proteolytic Enzymes and Heparin".
Journal of Clinical Endocrinology and Metabolism, vol. 56, No. 2, pp. 376-383, Feb. 1983, P. Chatelain, et al., "Effect of In Vitro Action of Serum Proteases or Exposure to Acid on Measurable Immunoreactive Somatomedin-C in Serum".
Horikawa Shuji
Kasahara Hiroyuki
Mori Kazumine
Takasu Shigeto
Daiichi Radioisotope Laboratories, Ltd.
Eyler Yvonne
Feisee Lila
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