Chemistry: analytical and immunological testing – Immunochemical pregnancy determination
Patent
1987-07-14
1989-07-25
Moskowitz, Margaret
Chemistry: analytical and immunological testing
Immunochemical pregnancy determination
436518, 436529, 436548, 436804, 436813, 436814, 436818, 436823, 935110, 435 7, 435 68, 4351722, 43524027, 435188, 435192, G01N 3376, G01N 33535, G01N 33543
Patent
active
048513563
ABSTRACT:
An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the .beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the .beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is delectable when both are bound to hCG.
In a presently preferred embodiment, an immunoassay for hCG or hCB.beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the .beta. subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the .beta. subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG.beta..
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Birken, S., Reprod. Processes Contracept., Chapter 24, pp. 529-534 (1981), McKerns, K. W. ed, Plenum Press, N.Y., N.Y.
Armstrong Elmo G.
Birken Steven
Canfield Robert E.
Ehrlich Paul H.
Moskowitz Margaret
The Trustees of Columbia University in the City of New York
White John P.
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